Journal: Cell reports

Article Title: Tissue-Resident PDGFRα + Progenitor Cells Contribute to Fibrosis versus Healing in a Context- and Spatiotemporally Dependent Manner

doi: 10.1016/j.celrep.2019.12.045

Figure Lengend Snippet: (A–F) Seven days after HLI induction and adoptive transfer of undifferentiated GFP+PDGFRα+ cells, we tracked injected cells by nuclear GFP expression and co-staining with anti-CD31 (A and B), anti-NG2 (C and D) and Cy3-conjugated anti-αSMA antibody (E and F). White arrowheads indicate GFP+ cells; yellow arrowheads in (B) show decreased expression of GFP. Images representative of n = 3 independent experiments and were obtained with a confocal Leica SP5 DM as z stacks.

Article Snippet: For flow cytometric analysis and FACS we used the following antibodies: APC-conjugated PDGFRα (eBioscience, #17-1401-81), APC-conjugated CD31 (BioLegend, #102409), APC-conjugated NG2 (R&D, #FAB2585A), PE-conjugated Sca1 (Becton Dickinson, #553108), PE-conjugated NG2 (Becton Dickinson #562415), APC-Cy7-conjugated CD45 (Becton Dickinson, #557659), PE-conjugated CD44 (eBioscience, 12-0441-82), PE-conjugated CD31 (Biolegend, #102408), PE-conjugated CD73 (Biolegend, #127205), PE-conjugated CD29 (Biolegend, #102207), PE-conjugated CD105 (R&D systems, #FAB1320P) and PE-conjugated PDGFRα (eBioscience 12-1402-81).

Techniques: Adoptive Transfer Assay, Injection, Expressing, Staining

Journal: Cell reports

Article Title: Tissue-Resident PDGFRα + Progenitor Cells Contribute to Fibrosis versus Healing in a Context- and Spatiotemporally Dependent Manner

doi: 10.1016/j.celrep.2019.12.045

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: For flow cytometric analysis and FACS we used the following antibodies: APC-conjugated PDGFRα (eBioscience, #17-1401-81), APC-conjugated CD31 (BioLegend, #102409), APC-conjugated NG2 (R&D, #FAB2585A), PE-conjugated Sca1 (Becton Dickinson, #553108), PE-conjugated NG2 (Becton Dickinson #562415), APC-Cy7-conjugated CD45 (Becton Dickinson, #557659), PE-conjugated CD44 (eBioscience, 12-0441-82), PE-conjugated CD31 (Biolegend, #102408), PE-conjugated CD73 (Biolegend, #127205), PE-conjugated CD29 (Biolegend, #102207), PE-conjugated CD105 (R&D systems, #FAB1320P) and PE-conjugated PDGFRα (eBioscience 12-1402-81).

Techniques: Recombinant, Derivative Assay, Sequencing, Staining, Software